In Vivo Analysis of Engrafted Adult Hippocampal Neural Progenitors

  • Matthew J. Robertson
  • Joseph Peltier
  • David V. Schaffer
Part of the Methods in Molecular Biology™ book series (MIMB, volume 621)


A neural degenerative disease is characterized by the deterioration of neural tissue and subsequent loss of function. The in vivo engraftment of neural stem cells is a promising approach to the functional replacement of neural tissue with the ultimate goal of regaining lost function. In addition, by studying the behavior of engrafted neural stem cells in healthy and diseased tissue, insight can be gained into the extracellular and intracellular mechanisms which regulate stem cell behavior in vivo. Adult hippocampal neural progenitor cells (AHNPCs) are one potential source of cells that can be used to this goal. In this chapter, we describe some of the in vivo techniques necessary to study hippocampal progenitors in the adult rat, including engraftment and analysis by immunofluorescent staining. These techniques are important for studying AHNPCs within the physiologically relevant environment of the adult brain rather than in a culture dish.

Key words

Neural Hippocampus Progenitors Rat In vivo Adult Engraftment Immunostaining Pulse-chase/BrdU Neurons Astrocytes Differentiation 


  1. 1.
    Gage FH, Coates PW, Palmer TD, Kuhn HG, Fisher LJ, Suhonen JO, Peterson DA, Suhr ST, Ray J (1995) Survival and differentiation of adult neuronal progenitor cells transplanted to the adult brain. Proc Natl Acad Sci U S A 92:11879–11883CrossRefPubMedGoogle Scholar
  2. 2.
    Suhonen JO, Peterson DA, Ray J, Gage FH (1996) Differentiation of adult hippocampus-derived progenitors into olfactory neurons in vivo. Nature 383:624–627CrossRefPubMedGoogle Scholar
  3. 3.
    Shihabuddin LS, Palmer TD, Gage FH (1999) The search for neural progenitor cells: prospects for the therapy of neurodegenerative disease. Mol Med Today 5:474–480CrossRefPubMedGoogle Scholar
  4. 4.
    Paxinos G, Watson C (1998) The rat brain in ste­reotaxic coordinates. Academic Press, San Diego, CAGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Matthew J. Robertson
    • 1
  • Joseph Peltier
    • 1
  • David V. Schaffer
    • 1
  1. 1.Department of Chemical EngineeringUniversity of CaliforniaBerkeleyUSA

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