Protocols for Adult Stem Cells pp 65-87

Part of the Methods in Molecular Biology™ book series (MIMB, volume 621)

In Vitro Culture and Analysis of Adult Hippocampal Neural Progenitors

  • Joseph Peltier
  • Smita Agrawal
  • Matthew J. Robertson
  • David V. Schaffer
Protocol

Abstract

Adult hippocampal neural progenitor cell (AHNPC) culture is a useful technique for gaining insight into adult neurogenesis, studying disease, and high throughput drug screening. The ability of AHNPCs to proliferate and differentiate into the three cell lineages of the adult brain in cell culture provides the researcher a powerful platform to study the extracellular and intracellular regulatory mechanisms in a well-controlled environment. In this chapter, we describe some of the in vitro techniques necessary to study hippocampal progenitors in the adult rat. This chapter details routine culture techniques for passaging and differentiating hippocampal progenitors. We also describe techniques for analyzing the culture state, such as proliferation and expression of cell fate markers by quantitative RT-PCR and immunofluorescence.

Key words

Neural Hippocampus Progenitors Rat In vitro Adult Quantitative RT-PCR Immunostaining Differentiation Proliferation Neurons Astrocytes 

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Joseph Peltier
    • 1
  • Smita Agrawal
    • 1
  • Matthew J. Robertson
    • 1
  • David V. Schaffer
    • 1
  1. 1.Department of Chemical EngineeringUniversity of CaliforniaBerkeleyUSA

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