Molecular Epidemiology of Microorganisms pp 71-87

Part of the Methods in Molecular Biology™ book series (MIMB, volume 551)

Molecular Genotyping of Microbes by Multilocus PCR and Mass Spectrometry: A New Tool for Hospital Infection Control and Public Health Surveillance

  • David J. Ecker
  • Christian Massire
  • Lawrence B. Blyn
  • Steven A. Hofstadler
  • James C. Hannis
  • Mark W. Eshoo
  • Thomas A. Hall
  • Rangarajan Sampath
Protocol

Abstract

We describe a new technology for the molecular genotyping of microbes using a platform known commercially as the Ibis T5000. The technology couples multilocus polymerase chain reaction (PCR) to electrospray ionization/mass spectrometry (PCR/ESI-MS) and was developed to provide rapid, high-throughput, and precise digital analysis of either isolated colonies or original patient specimens on a platform suitable for use in hospital or reference diagnostic laboratories or public health settings. The PCR/ESI-MS method measures digital molecular signatures from microbes, enabling real-time epidemiological surveillance and outbreak investigation. This technology will facilitate understanding of the pathways by which infectious organisms spread and will enable appropriate interventions on a time frame not previously achievable.

Key words

Molecular genotyping mass spectrometry PCR/ESI-MS base composition Ibis T5000 

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • David J. Ecker
    • 1
  • Christian Massire
    • 1
  • Lawrence B. Blyn
    • 1
  • Steven A. Hofstadler
    • 1
  • James C. Hannis
    • 1
  • Mark W. Eshoo
    • 1
  • Thomas A. Hall
    • 1
  • Rangarajan Sampath
    • 1
  1. 1.Ibis BiosciencesCarlsbadUSA

Personalised recommendations