Molecular Epidemiology of Microorganisms pp 159-171

Part of the Methods in Molecular Biology™ book series (MIMB, volume 551)

Comparison of Molecular Typing Methods Applied to Clostridium difficile

  • Ed J. Kuijper
  • Renate J. van den Berg
  • Jon S. Brazier
Protocol

Abstract

Since the 1980s the epidemiology of Clostridium difficile infection (CDI) has been investigated by the application of many different typing or fingerprinting methods. To study the epidemiology of CDI, a typing method with a high discriminatory power, typeability, and reproducibility is required. Molecular typing methods are generally regarded as having advantages over phenotypic methods in terms of the stability of genomic markers and providing greater levels of typeability. A growing number of molecular methods have been applied to C. difficile. For the early and rapid detection of outbreak situations, methods such as restriction enzyme analysis, arbitrary primed polymerase chain reaction (PCR), and PCR ribotyping are commonly used. For long-term epidemiology, multilocus sequence typing, multilocus variable number of tandem repeats analysis, and amplified fragment length polymorphism are of interest. Currently, the PCR-ribotyping method and the library of PCR ribotypes in Cardiff are the benchmarks to which most typing studies around the world are compared. Multilocus variable number of tandem repeats analysis is the most discriminative typing method and will contribute significantly to our understanding of the epidemiology of this important nosocomial pathogen.

Key words

Clostridium difficile MLVA PCR ribotyping REA subtyping 

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Ed J. Kuijper
    • 1
  • Renate J. van den Berg
    • 1
  • Jon S. Brazier
    • 1
  1. 1.Reference Laboratory for Clostridium difficile, Medical Microbiology Department, LUMC, LeidenThe National Institute for Public Health and EnvironmentBangkokThe Netherlands
  2. 2.Anaerobe Reference Laboratory, NPHS Microbiology CardiffUniversity Hospital of WalesHeath ParkUK

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