Mitosis pp 243-258

Part of the Methods in Molecular Biology book series (MIMB, volume 545)

The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and Recovery

  • Josefin Fernius
  • Kevin G. Hardwick
Protocol

Abstract

The spindle checkpoint is a surveillance mechanism that ensures the fidelity of chromosome segregation by inhibiting anaphase onset until all chromosomes have established stable bipolar attachments. Here we describe a number of protocols that can be used to assay the ability of budding and fission yeast cells to (1) establish and maintain a spindle checkpoint arrest, and (2) segregate chromosomes efficiently upon recovery from mitotic arrest. We focus on experimental detail of the budding yeast protocols, but also point out important differences between budding and fission yeast assays.

Key words

Checkpoint segregation recovery biorientation 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Josefin Fernius
    • 1
  • Kevin G. Hardwick
    • 1
  1. 1.Wellcome Trust Centre for Cell BiologyUniversity of EdinburghEdinburghUK

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