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Zebrafish pp 133-143 | Cite as

Nitroreductase-Mediated Cell Ablation in Transgenic Zebrafish Embryos

  • Harshan Pisharath
  • Michael J. Parsons
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 546)

Summary

Prodrug dependent cell ablation is a method that allows inducible and spatially restricted cell destruction. We describe transgenic methods to express the Escherichia coli nfsB in a tissue restricted manner in the zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme that can render prodrugs such as metronidazole (Met) cytotoxic. Using the expression of NTR fused to a fluorescent protein, one can simultaneously make cells susceptible to prodrug treatment and visualize cell ablation as it occurs.

Key words

Ablation Nitroreductase Metronidazole Prodrug Transactivation Gal4 UAS 

Notes

Acknowledgments

The authors wish to acknowledge Steve Leach for critical reading of the manuscript and Matthew Knabel for expert technical support. HP was supported by NIH NCRR T32 Grant 07002. MJP is supported in part by funding from the Juvenile Diabetes Research Foundation.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Department of Comparative MedicineJohns Hopkins School of MedicineBaltimoreUSA
  2. 2.Department of SurgeryJohns Hopkins School of MedicineBaltimoreUSA

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