Morphological Analysis of the Zebrafish Digestive System
The zebrafish provides an ideal model for the study of vertebrate organogenesis, including the formation of the digestive tract and its associated organs. Despite optical transparency of embryos, the internal position of the developing digestive system and its close juxtaposition with the yolk initially made morphological analysis relatively challenging, particularly during the first 3 d of development. However, methodologies have been successfully developed to address these problems and comprehensive morphologic analysis of the developing digestive system has now been achieved using a combination of light and fluorescence microscope approaches—including confocal analysis—to visualize wholemount and histological preparations of zebrafish embryos. Furthermore, the expanding number of antibodies that cross-react with zebrafish proteins and the generation of tissue-specific transgenic green fluorescent protein reporter lines that mark specific cell and tissue compartments have greatly enhanced our ability to successfully image the developing zebrafish digestive system.
Key wordsZebrafish Intestine Epithelium Goblet cells Enteroendocrine cells Histology Immunohistochemistry Transgenic lines
We would like to thank Val Feakes and Stephen Cody for assistance and advice with histology and imaging, respectively. Val Feakes, Annie Ng, Tanya de Jong-Curtain, Heather Verkade and Elsbeth Richardson are acknowledged for their role in protocol development. This work was supported by Project Grants (280916 and 433614) to JKH from the NHMRC, Australia. Adam Parslow is a Ludwig Institute Ph.D student in the Department of Surgery at the Royal Melbourne and Western Hospitals, University of Melbourne and a recipient of an Australian Postgraduate Award.
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