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DNA Replication

Volume 521 of the series Methods in Molecular Biology pp 55-70

Date:

Checkpoint Regulation of DNA Replication

  • Erik BoyeAffiliated withDepartment of Cell Biology, Institute for Cancer Research, Rikshospitalet-Radiumhospitalet Medical Centre Email author 
  • , Henriette C. Skjølberg
  • , Beáta Grallert

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Summary

We discuss the mechanisms regulating entry into and progression through S phase in eukaryotic cells. Methods to study the G1/S transition are briefly reviewed and an overview of G1/S-checkpoints is given, with particular emphasis on fission yeast. Thereafter we discuss different aspects of the intra-S checkpoint and introduce the main molecular players and mechanisms.

Key words

Checkpoints S phase DNA replication Gel electrophoresis Chromatin binding G1 phase