Tumor Biomarker Discovery pp 21-38

Part of the Methods in Molecular Biology book series (MIMB, volume 520)

Discovery of Antibody Biomarkers Using Protein Microarrays of Tumor Antigens Cloned in High Throughput

  • Madhumita Chatterjee
  • Jerzy Wojciechowski
  • Michael A. Tainsky
Protocol

Summary

Development of humoral and cellular immunity against self-cellular proteins in cancer patients is a phenomenal observation. The ability of immune system to sense the presence of the disease and to fight of the disease by generating autoantibodies against tumor antigens makes it a natural biosensor. Several screening technologies have been employed for the identification of tumor-specific antibodies in cancer patients. We have developed a multidimensional approach for the identification of diagnostic antigens that utilizes a combination of high-throughput antigen cloning and protein microarray-based serological detection of complex panels of antigens by exploiting the serum autoantibody repertoire directed toward tumor-associated antigens in cancer patients. Furthermore, validation of these antigens by different bioinformatics and biological approaches will reveal the diagnostic/prognostic utility of these antigens for personalized immunotherapy.

Keywords

Immune response Tumor biomarkers Autoantibodies Epitopes Protein microarrays Immunotherapy 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Madhumita Chatterjee
    • 1
  • Jerzy Wojciechowski
    • 1
  • Michael A. Tainsky
    • 1
  1. 1.Department of Molecular Biology and GeneticsBarbara Ann Karmanos Cancer Institute, Wayne State UniversityDetroitUSA

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