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Biosensors and Biodetection

Volume 503 of the series Methods in Molecular Biology™ pp 139-165

Label-Free Detection with the Liquid Core Optical Ring Resonator Sensing Platform

  • Ian M. WhiteAffiliated withBiological Engineering Department, University of Missouri-Columbia
  • , Hongying ZhuAffiliated withBiological Engineering Department, University of Missouri-Columbia
  • , Jonathan D. SuterAffiliated withBiological Engineering Department, University of Missouri-Columbia
  • , Xudong FanAffiliated withBiological Engineering Department, University of Missouri-Columbia
  • , Mohammed ZourobAffiliated withBiosensors Division

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Summary

Optical label-free detection prevents the cost and complexity of fluorescence and radio labeling while providing accurate quantitative and kinetic results. We have developed a new optical label-free sensor called the liquid core optical ring resonator (LCORR). The LCORR integrates optical ring resonator sensors into the microfluidic delivery system by using glass capillaries with a thin wall. The LCORR is capable of performing refractive index detection on liquid samples, as well as bio/chemical analyte detection down to detection limits on the scale of pg/mm2 on a sensing surface.

Keywords

Optical ring resonator LCORR Whispering gallery modes Refractive index detection Protease detection DNA sequence detection