Abstract
Current techniques in mass spectrometry (MS) allow sensitive and accurate identification of proteins thanks to the in silico availability of these protein sequences within databases.
This chapter provides a short overview of MS techniques used in the identification of phage structural proteins and focuses on an electron spray peptide ionization (ESI-MS/MS) approach to identify the phage structural proteome in a comprehensive and systematic ways. Such analyses provide an experimental examination of structural proteins and confirm genome-based gene predictions.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Lavigne, R., Ceyssens, PJ., Robben, J. (2009). Phage Proteomics: Applications of Mass Spectrometry. In: Clokie, M.R., Kropinski, A.M. (eds) Bacteriophages. Methods in Molecular Biology™, vol 502. Humana Press. https://doi.org/10.1007/978-1-60327-565-1_14
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DOI: https://doi.org/10.1007/978-1-60327-565-1_14
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