Chromatin Charting: Global Mapping of Epigenetic Effects

  • Chongyuan Luo
  • Eric Lam
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 553)

Abstract

To tackle the question of how chromatin organization is involved in global regulation of genome-related processes such as transcription, we have recently created a collection of 277 transposon-tagged Arabidopsis lines comprised of a single insert with a common luciferase reporter cassette and a LacO repeat array for visual tracking of the tagged region via fluorescent protein fusion technology. Using this collection of plants, one can begin to map transgene position effects as well as global epigenetic control in response to developmental or externally applied cues. In this chapter, we will outline the approach and methods for deploying this novel resource for the study of global gene control, using Arabidopsis as a convenient model system.

Key words

Chromatin transposon-tagged lines Arabidopsis epigenetics luciferase dsRNA suppression position effects 

Notes

Acknowledgments

This work is supported by an NSF Plant Genome Research Program grant to E.L. (#0077617).

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Chongyuan Luo
    • 1
  • Eric Lam
    • 1
  1. 1.Biotechnology Center for Agriculture and the EnvironmentRutgers UniversityNew BrunswickUSA

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