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Targeted Delivery of Antisense Oligonucleotides and siRNAs into Mammalian Cells

  • Mouldy Sioud Email author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 487)

Abstract

RNA interference (RNAi) is a natural mechanism for gene silencing that can be harnessed for the development of RNA-based drugs. Although synthetic small interfering RNA (siRNAs) can be delivered in vitro to virtually all cell types using lipid-based transfection agents or electroporation, efficient strategies for achieving either systemic or targeted delivery remains one of the major in vivo challenges. Among the targeting strategies, receptor-targeted delivery provides an innovative strategy to selectively direct therapeutics to cancer cells. Receptor-binding peptides can be incorporated into gene-delivery vesicles or directly conjugated to siRNAs in the hope of promoting their localization in target cells expressing the cognate receptors. This chapter discusses the current status of siRNA-targeting strategies using either peptides identified through iterative screening of random peptide phage libraries or naturally occurring peptides. Also, transcriptional targeting strategies and detailed protocols for the selection of cancer cell-binding peptide from random peptide phage libraries are described.

Keywords

RNAi siRNA random peptide libraries hormone peptides peptide analogues endocytose cell surface receptors 

Notes

Acknowledgments

We thank Dr Anne Dybwad for critical reading of the manuscript and the group members for their contribution to this work.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Institute for Cancer Research, Department of ImmunologyMolecular Medicine Group, M. Sioud (馓)MontebelloNorway

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