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Reporter Gene Assays

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Cell-Based Assays for High-Throughput Screening

Part of the book series: Methods in Molecular Biology ((MIMB,volume 486))

Summary

Reporter gene assays are versatile and sensitive methods of assaying numerous targets in high-throughput drug-screening programs. A variety of reporter genes allow users a choice of signal that can be tailored to the required sensitivity, the available detection apparatus, the cellular system employed, and the required compatibility with multiplexed assays. Promoters used to drive reporter gene expression can be activated either by a broad range of biochemical pathways or by the selective activation of individual targets. In this chapter, we will introduce some of the considerations behind the choice of reporter gene assays and describe the methods that we have used to establish 96-well format luciferase and aequorin assays for the screening of ligands for G protein-coupled receptors.

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Acknowledgments

This work was supported in part by the Hong Kong Jockey Club and grants from the Research Grants Council of Hong Kong (HKUST 6420/05M) and the University Grants Committee (AoE/B-15/01). YHW was a recipient of the Croucher Senior Research Fellowship.

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Authors and Affiliations

  1. Department of Biochemistry, Molecular Neuroscience Center, Biotechnology Research Institute, Hong Kong University of Science and Technology, Hong Kong, China

    Andy M. F. Liu, David C. New, Rico K. H. Lo & Yung H. Wong

Authors
  1. Andy M. F. Liu
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  2. David C. New
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  3. Rico K. H. Lo
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  4. Yung H. Wong
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Corresponding author

Correspondence to Yung H. Wong .

Editor information

Editors and Affiliations

  1. Broad Institute of Harvard, Chemical Biology Program, Massachusetts Institute of Technology, Cambridge Center 7, Cambridge, 02142, U.S.A.

    Paul A. Clemons

  2. Broad Institute of Harvard, Chemical Biology Program, Massachusetts Institute of Technology, Cambridge Center 7, Cambridge, 02142, U.S.A.

    Nicola J. Tolliday

  3. Broad Institute of Harvard, Chemical Biology Program, Massachusetts Institute of Technology, Cambridge Center 7, Cambridge, 02142, U.S.A.

    Bridget K. Wagner

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Liu, A.M.F., New, D.C., Lo, R.K.H., Wong, Y.H. (2009). Reporter Gene Assays. In: Clemons, P., Tolliday, N., Wagner, B. (eds) Cell-Based Assays for High-Throughput Screening. Methods in Molecular Biology, vol 486. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-545-3_8

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  • DOI: https://doi.org/10.1007/978-1-60327-545-3_8

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-544-6

  • Online ISBN: 978-1-60327-545-3

  • eBook Packages: Springer Protocols

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