Assay for Isolation of Inhibitors of Her2-Kinase Expression

  • Gabriela Chiosis
  • Adam B. Keeton
Part of the Methods in Molecular Biology book series (MIMB, volume 486)


Her2 (ErbB2) protein is overexpressed in breast and other solid tumors, and its expression is associated with progressive disease. Current therapies directed toward Her2 either block dimerization of the receptor or inhibit tyrosine kinase activity to disrupt intracellular signaling. However, little is known about alternative mechanisms for suppressing Her2 expression, possibly by inducing degradation or blocking synthesis. Here, we describe a hybrid western-blotting and enzyme-linked immunosorbent assay (ELISA) designed to identify in low- to medium-throughput format noncytotoxic compounds that reduce expression of Her2 protein.

Key words

Breast cancer Heat-shock protein 90 (Hsp90) Her2 (ErbB2) tyrosine kinase Whole-cell immunoblot 



The author would like to thank Joungnam Kim and Cristina C. Clement for technical assistance. This work was supported by R03 MH076408, and a Susan G. Komen grant, R01-SGK-BCTR0504381.


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Gabriela Chiosis
    • 1
  • Adam B. Keeton
    • 2
  1. 1.Program in Molecular Pharmacology and ChemistryMemorial Sloan-Kettering Cancer CenterNew YorkUSA
  2. 2.Southern Research Molecular Libraries Screening Center, Assay Implementation GroupSouthern Research InstituteBirminghamUSA

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