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Analysis of Frequency and Phenotype of Antigen-Specific T Cells

  • Angus Stock
  • Vincenzo Cerundolo
Part of the Methods in Molecular Biology™ book series (MIMB, volume 514)

Abstract

Over the last decade, our understanding of the cellular immune system has been greatly advanced through the development of methods to identify antigen-specific T cells directly ex vivo. The major reagents and techniques used for this purpose are (i) tetramerised MHC:peptide complexes (tetramers) which bind to specific T-cell receptors (TCR) and (ii) assays that detect T cells which synthesise cytokines in response to cognate stimulation (intracellular cytokine staining (ICS)). Here, we provide a detailed description of the procedure for generating and using class I MHC:peptide tetramers to label peptide-specific T cells and for carrying out ICS to measure antigen-specific T lymphocytes.

Key words

Tetramers antigen-specific T cells MHC class I intracellular cytokine staining CTL 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Angus Stock
    • 1
  • Vincenzo Cerundolo
    • 1
  1. 1.Nuffield Department of Clinical MedicineWeatherall Institute of Molecular MedicineUK

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