Application of lacZ Transgenic Mice to Cell Lineage Studies
Cell lineage analyses trace the hierarchy of cell types derived from a progenitor population. Critical to these analyses is the ability to track reliably all or defined subsets of the clonal descendants of the progenitor population. This necessitates marking the cells with a heritable and cell autonomous marker. Transgenes encoding molecules that can be visualized directly in situ without compromising cell differentiation, such as the reporter, β-galactosidase encoded by lacZ and chloramphenicol acetyltransferase, encoded by the CAT gene are the most widely used. The lacZ can be readily detected using a sensitive histo-chemical assay such that cells in which the β-galactosidase gene is transcrip-tionally active produce a blue stain in tissue sections or in whole mounts (1). Some lineage studies demand the simultaneous detection of the lacZ product and other tissue-specific proteins or transcripts. In this chapter, we discuss the experimental strategies in which the lacZ...
KeywordsPhosphate Buffer Saline Canada Balsam Prehybridization Solution Free Phosphate Buffer Saline Alkaline Phosphatase Buffer
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