The Nucleus pp 267-283 | Cite as

Detection of the Nuclear Poly(ADP-ribose)-Metabolizing Enzymes and Activities in Response to DNA Damage

  • Jean-Christophe Amé
  • Antoinette Hakmé
  • Delphine Quenet
  • Elise Fouquerel
  • Françoise Dantzer
  • Valérie Schreiber
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 464)

Abstract

Poly(ADP-ribosyl)ation is a posttranslational modification of proteins in higher eukaryotes mediated by poly(ADP-ribose) polymerases (PARPs) that is involved in many physiological processes such as DNA repair, transcription, cell division, and cell death. Biochemical studies together with PARP-1- or PARP-2-deficient cellular and animal models have revealed the redundant but also complementary functions of the two enzymes in the surveillance and maintenance of genome integrity. Poly(ADP-ribose) is degraded by the endo- and exo-glycosidase activities of poly(ADP-ribose) glycohydrolase (PARG). In this chapter, biochemical and immunofluorescence methods are described for detecting and assaying PARPs and PARG.

Keywords

Poly(ADP-ribose) Poly(ADP-ribose) polymerases Poly(ADP-ribose) glycohydrolase DNA damage Genome integrity Western blotting Immunofluorescence 

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Copyright information

© Humana Press, a part of Springer Science + Business Media, LLC 2008

Authors and Affiliations

  • Jean-Christophe Amé
    • 1
  • Antoinette Hakmé
    • 1
  • Delphine Quenet
    • 1
  • Elise Fouquerel
    • 1
  • Françoise Dantzer
    • 1
  • Valérie Schreiber
    • 1
  1. 1.Département Intégrité du GénomeUMR 7175-LCI du CNRS École Supérieure de Biotechnologie de StrasbourgIllkirchFrance

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