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Bioinformatics pp 245-255 | Cite as

Expression and Microarrays

  • Joaquín Dopazo
  • Fátima Al-Shahrour
Part of the Methods in Molecular Biology™ book series (MIMB, volume 453)

Abstract

High throughput methodologies have increased by several orders of magnitude the amount of experimental microarray data available. Nevertheless, translating these data into useful biological knowledge remains a challenge. There is a risk of perceiving these methodologies as mere factories that produce never-ending quantities of data if a proper biological interpretation is not provided.

Methods of interpreting these data are continuously evolving. Typically, a simple two-step approach has been used, in which genes of interest are first selected based on thresholds for the experimental values, and then enrichment in biologically relevant terms in the annotations of these genes is analyzed in a second step. For various reasons, such methods are quite poor in terms of performance and new procedures inspired by systems biology that directly address sets of functionally related genes are currently under development.

Key words

Functional interpretation functional genomics multiple testing gene ontology 

Notes

Acknowledgments

This work is supported by grants from MEC BIO2005-01078, NRC Canada-SEPOCT Spain, and Fundación Genoma España.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Joaquín Dopazo
    • 1
  • Fátima Al-Shahrour
    • 1
  1. 1.Department of BioinformaticsCentro de Investigación Príncipe Felipe (CIPF)ValenciaSpain

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