The Fast Chromatin Immunoprecipitation Method

  • Joel Nelson
  • Oleg Denisenko
  • Karol Bomsztyk
Part of the Methods in Molecular Biology book series (MIMB, volume 567)


The chromatin immunoprecipitation assay (ChIP assay) has greatly facilitated the recent, dramatic expansion of our knowledge of the protein–DNA interactions involved in regulating gene expression, DNA repair, and cell division. The power of the assay is that it gives a researcher the ability to not only detect a specific protein–DNA interaction in vivo but also determine the relative density of factors along genes or the entire genome. Though powerful, the traditional assay is time consuming (involving 2 days or more) and laborious. With Fast ChIP, we simplified the assay to greatly reduce the time and labor involved. The improved assay is especially useful for studies which involve many samples, including the probing of multiple chromatin factors simultaneously and/or looking at genomic events over several time points. Using Fast ChIP, 24 sheared chromatin samples can be processed to yield PCR-ready DNA in 5 h.

Key words

Chromatin immunoprecipitation ChIP-chip tissue ChIP transcription DNA repair 



We thank members of the KB lab for valuable discussions of the method. This work was supported by NIH DK45978 and GM45134 to K.B.


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Joel Nelson
    • 1
  • Oleg Denisenko
    • 2
  • Karol Bomsztyk
    • 1
    • 2
  1. 1.Molecular and Cellular Biology ProgramUniversity of WashingtonSeattleUSA
  2. 2.UW Medicine at Lake UnionUniversity of WashingtonSeattleUSA

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