Preparation of Tissue and Sections

  • Janet V. Passonneau
  • Oliver H. Lowry
Part of the Biological Methods book series (BM)


The following portion of this book represents a coherent system of quantitative analysis for histochemical purposes. It derives from the original system of Linderstrom-Lang and Holter (1), but differs in several significant details. In the original methodology of the Carlsberg Laboratory, serial frozen sections were cut from a uniform tissue block. Alternate sections were either analyzed, or fixed and stained for histological control. This procedure has been further developed and exploited with great success by David Glick, and is fully described in book form (2).


Pyridine Nucleotide Dewar Flask Serial Freeze Section Vapor Tension Histological Control 
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  1. 1.
    Linderstrom-Lang, K., Holter, H., and Ohlsen, A. S. (1935) Studies on enzymatic histochemistry XIII. The distribution of enzymes in the stomach of pigs. Compt.-Rend. Lab. Carlsberg, Serie Chim. 20, 66–125.Google Scholar
  2. 2.
    Glick, D. (1961,1963) Quantitive Chemical Techniques of Histo-and Cytochemistry, vols. 1 and 2 (Wiley [Interscience]), New York.Google Scholar
  3. 3.
    Cole, B. R., Boylan, J. G., Bross, T. E., Burch, H. B., and Lowry, O. H. (1988) Progressive enzyme changes within anatomically defined segments of rat nephron: Demonstration of anew technique. J. Histochem. Cytochem. 36, 285–289.PubMedGoogle Scholar
  4. 4.
    Barbehenn, E. K., Wales, R. G., and Lowry, O. H. (1974) The explanation for the blockade of glycolysis in early mouse embryos. Proc. Natl. Acad. Sci. USA 71, 1056–1060.PubMedCrossRefGoogle Scholar
  5. 5.
    Chi, M. M.-Y., Manchester, J. K., Yang, V. C., Curato, A. D., Strickler, R. C., and Lowry, O. H. (1988) Contrast in levels of metabolic enzymes in human and mouse ova. Biol. of Reprod. 39, 295–307.CrossRefGoogle Scholar
  6. 6.
    Outlaw, W. H., Jr. and Lowry, O. H. (1977) Organic acid and potassium accumulation in guard cells during stomatal opening. Proc. Natl. Acad Sci. USA 74, 4434–4438.PubMedCrossRefGoogle Scholar
  7. 7.
    Wenger, B. (1955) Personal communication.Google Scholar
  8. 8.
    Essén, B. Jansson, E., Henriksson, J., Taylor, A. W., and Saltin, B. (1975) Metabolic characteristics of fibre types in human skeletal muscle. Acta Physiol. Scand. 95, 153–165.PubMedCrossRefGoogle Scholar
  9. 9.
    Lowry, C. V., Kimmey, J. S., Felder, S., Chi, M. M.-Y., Kaiser, K. K., Passonneau, P. N., Kirk, K. A., and Lowry, O. H. (1978) Enzyme patterns in single human muscle fibers. J. Biol. Chem. 253, 8269–8277.PubMedGoogle Scholar
  10. 10.
    Hintz, C. S., Lowry, C. V., Kaiser, K. K., McKee, D., and Lowry, O. H. Enzyme levels in individual rat muscle fibers. Am. J. Physiol. 239, C58–C65.Google Scholar

Copyright information

© The Humana Press Inc. 1993

Authors and Affiliations

  • Janet V. Passonneau
    • 1
  • Oliver H. Lowry
    • 2
  1. 1.WashingtonUSA
  2. 2.Washington UniversitySt. LouisUSA

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