Enzymatic Cycling

  • Janet V. Passonneau
  • Oliver H. Lowry
Part of the Biological Methods book series (BM)


Biochemists have an insatiable demand for analytical sensitivity. This is because some of the most interesting biological materials are present in tissues at very low concentrations and some crucial biochemical questions concern amounts of materials in single cells or parts of cells. In many (perhaps most) cases, the needed sensitivity can be provided by methods based on radioactive labeling. There are, nevertheless, other situations in which tracer methods are not sensitive enough, are difficult to apply, or a direct chemical assay would simply take less time. For example, it might be difficult to measure the concentration of glucose-6-P in a microgram of tissue with radioactive methods. With enzymatic cycling, this is no problem.


Pyruvate Kinase Pyridine Nucleotide Cycling Rate Leuconostoc Mesenteroides Indicator Reaction 
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  1. 1.
    Cha, S. and Cha, C.-J. M. (1965) Kinetics of cyclic enzyme systems. Mol. Pharmacol. 1, 178,179.PubMedGoogle Scholar
  2. 2.
    Hintz, C. S., Chi, M. M.-Y., and Lowry, O. H. (1980) Correcting potential defect in an enymatic cycle for NADP. Anal. Biochem. 128, 186–190.CrossRefGoogle Scholar
  3. 3.
    Lowry, O. H., Passonneau, J. V., Schulz, D. W., and Rock, M. K. (1961) The measurement of pyridine nucleotides by enzymatic cycling. J. Biol. Chem. 236, 2659–2756.Google Scholar
  4. 4.
    Chi, M. M.-Y., Lowry, C. V., and Lowry, O. H. (1978) An improved enzymatic cycle for nicotinamide adenine dinucleotide phosphate. Anal. Biochem. 89, 119–129.PubMedCrossRefGoogle Scholar
  5. 5.
    Kato, T., Berger, S. J., Carter, J. A., and Lowry, O. H. (1973) An enzymatic cycling method for nicotinamide-adenine dinucleotide with malic and alcohol dehydrogenases. Anal. Biochem. 53, 86–97.PubMedCrossRefGoogle Scholar
  6. 6.
    Breckenridge, B. McL. (1964) The measurement of cyclic adenlyate in tissues. Proc. Natl. Acad. Sci. USA 52, 1580–1586.PubMedCrossRefGoogle Scholar
  7. 7.
    Goldberg, N. D., Dietz, S. B., and O’Toole, A. G. (1969) Cyclic guanosine 3′,5′ monophosphate in mammalian tissues and urine. J. Biol. Chem. 224, 4458–4466.Google Scholar

Copyright information

© The Humana Press Inc. 1993

Authors and Affiliations

  • Janet V. Passonneau
    • 1
  • Oliver H. Lowry
    • 2
  1. 1.WashingtonUSA
  2. 2.Washington UniversitySt. LouisUSA

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