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Histochemical Analyses

  • Janet V. Passonneau
  • Oliver H. Lowry
Protocol
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Part of the Biological Methods book series (BM)

Abstract

Analyses of histological samples are made by the same basic principles described for larger specimens. Samples of course will generally be small, and may become very minute as the analyst’s ambition grows to study smaller and smaller structures. This implies a demand for an enormous range of sensitivity for studies, all of which could be denoted as quantitative histochemistry or cytochemistry. Consequently, in adapting methods to histochemical purposes, certain modifications must be introduced depending on the sample size and the substance concerned. To measure lactate dehydrogenase in a 2-µg sample from the molecular layer of cerebellum requires a million times less sensitivity than to measure lactate in a 2-ng portion of an anterior horn cell. To measure the lactate dehydrogenase, it is easy to provide sufficient sensitivity, but for reasons of convenience, the reaction is carried out at high tissue dilution. This makes it necessary to be concerned about the effects of dilution on enzyme stability. To measure the lactate requires not only great sensitivity, but also the use of very small volumes for the reaction in order to circumvent blank problems.

Keywords

Anterior Horn Cell Silicone Coating Aqueous Droplet Surface Denaturation Wooden Stand 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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    Lowry, O. H. (1963) The chemical study of single neurons. Harvey Lect. 58, 1–19.PubMedGoogle Scholar
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    Matschinsky, F. M., Passonneau, J. V., and Lowry, O. H. (1968a) Quantitative histochemical analysis of glycolytic intermediates and cofactors with an oil well technique. J. Histochem. Cytochem. 16, 29–39.PubMedGoogle Scholar
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    Matschinsky, F. M., Rutherford, C. R., and Ellerman, J. E. (1968) Accumulation of citrate in pancreatic islets of obese hyperglycemic mice. Biochem. Biophys. Res. Commun. 33, 855–862.PubMedCrossRefGoogle Scholar

Copyright information

© The Humana Press Inc. 1993

Authors and Affiliations

  • Janet V. Passonneau
    • 1
  • Oliver H. Lowry
    • 2
  1. 1.WashingtonUSA
  2. 2.Washington UniversitySt. LouisUSA

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