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Flow Cytometric Studies on Intracellular Drug Fluorescence

  • Awtar Krishan
Protocol
Part of the Biological Methods book series (BM)

Abstract

Flow cytometry has rapidly evolved from a technique for detecting and sorting cells on the basis of their DNA content or immunological markers into a useful tool for detection and quantitation of intracellular drug fluorescence (2,25,30,37). Recent studies have shown that intracellular content of fluorescent drugs can be rapidly quantitated on a cell-to-cell basis by this sophisticated analytical method. Thus, one can analyze intracellular drug transport (influx, efflux), retention, and/or binding, and correlate these parameters with effects on cellular metabolism and proliferation (21,22).

Keywords

Drug Transport Soft Agar Assay Intracellular Fluorescence Forward Angle Light Scatter Semisynthetic Analog 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Humana Press Inc. 1987

Authors and Affiliations

  • Awtar Krishan
    • 1
  1. 1.Comprehensive Cancer Center and Department of OncologyUniversity of Miami Medical SchoolMiami

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