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Assay of Cell Cycle Kinetics by Multivariate Flow Cytometry Using the Principle of Stathmokinesis

  • Zbigniew Darzynkiewicz
  • Frank Traganos
  • Marek Kimmel
Protocol
Part of the Biological Methods book series (BM)

Abstract

Many of the existing cytokinetic techniques are based on the use of radioisotope-labeled DNA precursors (see  Chapter 1 Chapter 3 of this volume). Although numerous data obtained by these techniques contributed greatly to our present knowledge of the cell cycle, there are certain disadvantages related to the use of isotopes in general that limit their wider application, especially in the clinic. The drawbacks of autoradiography, which requires long exposure times and cumbersome grain-count analysis, are well known. There are also problems with the quantitative aspect of the autoradiography; especially of tritium detection (e.g., see ref 51). The alternative of autoradiography, liquid scintillation spectroscopy, precludes analysis of individual cells and thus restricts studies on cell heterogeneity. The variability in pools of endogenous nucleotides and changes in the accessibility of the precursor in vivo or in vitro create additional problems when any quantitative data on the rate of DNA replication (progression through S-phase) must be obtained based on radioactivity measurements. The most important limitation, however, especially when tritium-labeled thymidine is used, is the radiobiological effect of the precursor.

Keywords

Acridine Orange Cell Arrest Cell Progression Flow Cytometric Measurement Mitotic Inhibitor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Humana Press Inc. 1987

Authors and Affiliations

  • Zbigniew Darzynkiewicz
    • 1
  • Frank Traganos
    • 1
  • Marek Kimmel
    • 1
  1. 1.Walker LaboratorySloan-Kettering Institute of Cancer ResearchRye

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