• Felix J. Frey
  • Brigitte M. Frey
  • Leslie Z. Benet
Part of the Biological Methods book series (BM)


If a radioimmunoassay, a protein binding method, or a colorimetric assay for the assessment of a steroid level is replaced by high performance liquid chromatography (HPLC), the cost for the determination of a steroid level increases at least initially because one must acquire the new HPLC equipment. Therefore, if an older method provides the same results as the new, “advanced” HPLC method, the only advantage resulting from the introduction of a high performance chromatographic assay is that gained by the manufacturer in terms of greater sales. Thus, justification for the assessment of steroids by HPLC is only obtained if the quality and/or quantity of information gained is significantly increased as compared to that provided by the conventional methods. But this evidential relation, that more and better information justifies a higher price in any case, is no longer true in health care, with the birth some years ago of the categoric imperative for the reduction of costs in the medical sector. That is, each new technology introduced for health maintenance should demonstrate at least a stabilizing impact on total medical expenditures. Therefore, after reviewing the presently available HPLC methods for the clinically important steroids, we will consider whether HPLC analyses for these steroids can be recommended without violating this vox populi.


High Performance Liquid Chromatography High Performance Liquid Chromatography Bile Acid Congenital Adrenal Hyperplasia Chenodeoxycholic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© The Humana Press, Inc. 1981

Authors and Affiliations

  • Felix J. Frey
    • 1
  • Brigitte M. Frey
    • 1
  • Leslie Z. Benet
    • 1
  1. 1.Division of Clinical Pharmacology Department of Medicine and Department of PharmacyUniversity of CaliforniaSan Francisco

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