Principles of Liquid Chromatography

  • Stephen R. Bakalyar
Part of the Biological Methods book series (BM)


This article reviews the basic principles of high performance liquid chromatography (HPLC). The introductory section provides an overview of the HPLC technique, placing it in historical context and discussing the elementary facts of the separation mechanism. The next section discusses the nature of resolution, describing the two principal aspects, zone center separation and zone spreading. The third section takes a detailed look at how HPLC is used in practice to achieve a separation. It discusses the three key variables that need to be adjusted: retention, efficiency, and selectivity. A fourth section is concerned with various relationships of practical importance: flow rate, temperature, and pressure. A final section discusses future trends in HPLC.


High Performance Liquid Chromatography Mobile Phase High Performance Liquid Chromatography Column Length Plate Height 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Majors, R. E., J. Chromatogr. Sci. 15, 334 (1977).CrossRefGoogle Scholar
  2. 2.
    Bakalyar, S. R., Amer. Lab. 10, 43 (1978).Google Scholar
  3. 3.
    Karger, B. L., Gant, J. R., Hartkopf, A., and Weiner, P. H., J. Chromatogr. 128, 65 (1976).CrossRefGoogle Scholar
  4. 4.
    Bakalyar, S. R., McIlwrick, R., and Roggendorf, E., J. Chromatogr. 142, 353, (1977).CrossRefGoogle Scholar
  5. 5.
    Giddings, J. C., Dynamics of Chromatography, Part I, Dekker, New York, 1965.Google Scholar

Copyright information

© The Humana Press, Inc. 1981

Authors and Affiliations

  • Stephen R. Bakalyar
    • 1
  1. 1.Rheodyne, Inc.Berkeley

Personalised recommendations