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A Designed Peptide Chip: Protein Fingerprinting Technology with a Dry Peptide Array and Statistical Data Mining

  • Kenji Usui
  • Kin-ya Tomizaki
  • Hisakazu Mihara
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 570)

Abstract

There has recently been increased interest in the potential for microarray technologies to study protein networks in a whole cell system within a single experiment. Protein-detecting microarrays are composed of numerous agents immobilized within a tiny area on solid surfaces to capture targeted proteins and to detect interactions in a high-throughput fashion. In this chapter, in order to extend the usability of peptide microarrays, we describe a novel dry peptide microarray format to obtain protein fingerprint (PFP) data sets and a statistical PFP data manipulation technique to quantitatively analyze targeted proteins.

Key words

Protein-detecting chip designed peptide dry peptide array fluorimetric assay statistical data mining 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Kenji Usui
    • 1
  • Kin-ya Tomizaki
    • 2
  • Hisakazu Mihara
    • 1
  1. 1.Graduate School of Bioscience and BiotechnologyTokyo Institute of TechnologyYokohamaJapan
  2. 2.Innovative Materials and Processing Research Center and Department of Materials ChemistryRyukoku UniversitySetaJapan

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