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Detection of Ovarian Matrix Metalloproteinase mRNAs by In Situ Hybridization

  • Katherine L. Rosewell
  • Thomas E. CurryJr
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 590)

Abstract

In situ hybridization represents a powerful technique to localize DNA or RNA of interest at the chromosomal or cellular level. In endocrine tissues composed of diverse and varied cell types, in situ hybridization has allowed the identification of specific cells responsible for the expression of genes controlling the function of the tissue. Our laboratory has routinely used this approach to understand the cellular expression of genes associated with the growth of the ovarian follicle, rupture of the follicle, and transformation of the ruptured follicle into the corpus luteum. The current study outlines the procedural details of in situ detection of mRNA in tissues and illustrates the utility of this approach in identifying the ovarian cells expressing the matrix metalloproteinases and their endogenous inhibitors, the TIMPs, in the human ovary.

Key words

Ovary in situ hybridization matrix metalloproteinase TIMP human 

Notes

Acknowledgements

We are indebted to Ms. Sarah Wheeler-Price and Ms. Lauren Thomas for their assistance with the in situ hybridization. This work was supported by grants to TEC (NIH HDO57446 and NCRR P20 RR15592).

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Katherine L. Rosewell
    • 1
  • Thomas E. CurryJr
    • 1
  1. 1.Department of Obstetrics and GynecologyUniversity of Kentucky College of MedicineLexingtonUSA

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