Detection of Ovarian Matrix Metalloproteinase mRNAs by In Situ Hybridization
In situ hybridization represents a powerful technique to localize DNA or RNA of interest at the chromosomal or cellular level. In endocrine tissues composed of diverse and varied cell types, in situ hybridization has allowed the identification of specific cells responsible for the expression of genes controlling the function of the tissue. Our laboratory has routinely used this approach to understand the cellular expression of genes associated with the growth of the ovarian follicle, rupture of the follicle, and transformation of the ruptured follicle into the corpus luteum. The current study outlines the procedural details of in situ detection of mRNA in tissues and illustrates the utility of this approach in identifying the ovarian cells expressing the matrix metalloproteinases and their endogenous inhibitors, the TIMPs, in the human ovary.
Key wordsOvary in situ hybridization matrix metalloproteinase TIMP human
We are indebted to Ms. Sarah Wheeler-Price and Ms. Lauren Thomas for their assistance with the in situ hybridization. This work was supported by grants to TEC (NIH HDO57446 and NCRR P20 RR15592).
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