Transgenic Mouse Technology: Principles and Methods

  • T. Rajendra Kumar
  • Melissa Larson
  • Huizhen Wang
  • Jeff McDermott
  • Illya Bronshteyn
Part of the Methods in Molecular Biology book series (MIMB, volume 590)


Introduction of foreign DNA into the mouse germ line is considered a major technical advancement in the fields of developmental biology and genetics. This technology now referred to as transgenic mouse technology has revolutionized virtually all fields of biology and provided new genetic approaches to model many human diseases in a whole animal context. Several hundreds of transgenic lines with expression of foreign genes specifically targeted to desired organelles/cells/tissues have been characterized. Further, the ability to spatio-temporally inactivate or activate gene expression in vivo using the “Cre-lox” technology has recently emerged as a powerful approach to understand various developmental processes including those relevant to molecular endocrinology. In this chapter, we will discuss the principles of transgenic mouse technology, and describe detailed methodology standardized at our institute.

Key words

Transgene mouse embryo pseudopregnancy microinjection gene manipulation reporter genes integration of foreign DNA 



T.R.K. acknowledges funding support from NIH (NCRR Center for Biomedical Research Excellence Program Grant RR024214, HD04394, HD056082 and K-INBRE P20 RR016475) and The Hall Family Foundation, Kansas City, MO. H.W. is the recipient of a postdoctoral fellowship from the Biomedical Research Training Program at the Kansas University Medical Center.


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • T. Rajendra Kumar
    • 1
  • Melissa Larson
    • 2
  • Huizhen Wang
    • 3
  • Jeff McDermott
    • 4
  • Illya Bronshteyn
    • 4
  1. 1.Departments of Molecular & Integrative Physiology, Pathology and Laboratory Medicine, and Institute of Maternal and Fetal Biolgoy, Center for Reproductive SciencesUniversity of Kansas Medical CenterKansas CityUSA
  2. 2.Department of Molecular and Integrative PhysiologyUniversity of Kansas Medical CenterKansas CityUSA
  3. 3.Department of Molecular and Integrative Physiology, Center for Reproductive SciencesUniversity of Kansas Medical CenterKansas CityUSA
  4. 4.Transgenic and Gene-Targeting Institutional FacilityUniversityof Kansas Medical CenterKansas CityUSA

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