Laser Microdissection

  • Graeme I. Murray
Part of the Springer Protocols Handbooks book series (SPH)

1. Introduction

Solid tissues and organs, especially diseased tissues, are complex structures composed of heterogeneous mixtures of morphologically and functionally distinct cell types. The purposeful molecular study of cytologically and/or phenotypically specific cell types from tissues either normal or often more importantly abnormal or diseased requires the availability of rapid, efficient and accurate methods for obtaining specific defined groups of cells for further study. Whereas for example circulating blood cells can readily be separated into their distinct morphological and phenotypic classes by cell sorting using flow cytometry obtaining specific types of cells from solid tissues for analysis has until recently been much more difficult. Although manual methods of tissue microdissection have been described they are slow, cumbersome and not very specific. However, the development of laser based methods of microdissection for selecting specific types of cells from thin sections...


Laser Capture Microdissection Fixed Tissue Plastic Membrane Microdissected Tissue Unfixed Tissue 
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  1. 1.
    Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, Goldstein SR, Weiss RA, Liotta LA (1996) Laser capture microdissection. Science 274:998– 1001PubMedCrossRefGoogle Scholar
  2. 2.
    Simone NL, Bonner RF, Gillespie JW, Emmert- Buck MR, Liotta LA (1998) Laser-capture microdissection: opening the microscopic frontier to molecular analysis. Trends Genet 14:272–276PubMedCrossRefGoogle Scholar
  3. 3.
    Arcturus BioScience. accessed on 12th May 2006
  4. 4.
    Curran S, Murray GI (2005) An introduction to laser based tissue microdissection techniques. In: Murray GI, Curran S (eds) Methods in molecular biology vol 293: Laser capture microdissection methods and protocols. Humana, Totowa, NJ, pp 3–8Google Scholar
  5. 5.
    Curran S, McKay JA, McLeod HL, Murray GI (2000) Laser capture microscopy. Mol Pathol 53:64–68PubMedCrossRefGoogle Scholar
  6. 6.
    Curran S, Murray GI (2002) Tissue microdissection and its' applications in pathology. Current Diagn Pathol 8:183–192CrossRefGoogle Scholar
  7. 7.
    Dundas SR, Curran S, Murray GI (2002) Laser capture microscopy: application to urological cancer research. UroOncology 2:33–35CrossRefGoogle Scholar
  8. 8.
    Murray GI, Curran S (eds) Methods in molecular biology vol. 293: Laser capture microdissection methods and protocols. Humana, Totowa, NJGoogle Scholar
  9. 9.
    Gjerdrum LM, Lielpetere I, Rasmussen LM, Bendix K, Hamilton-Dutoit S (2001) Laser-assisted microdissection of membrane-mounted paraffin sections for polymerase chain reaction analysis: identification of cell populations using immu-nohistochemistry and in situ hybridization. J Mol Diagn 3:105–110PubMedCrossRefGoogle Scholar
  10. 10.
    Bohm M, Wieland I, Schutze K, Rubber H (1997) Microbeam MOMeNT: non-contact laser microdissection of membrane-mounted native tissue. Am J Pathol 151:63–67PubMedGoogle Scholar
  11. 11.
    Schutze K, Lahr G (1998) Identification of expressed genes by laser-mediated manipulation of single cells. Nat Biotechnol 16:737–742PubMedCrossRefGoogle Scholar
  12. 12.
    Lawrie LC, Curran S, McLeod HL, Fothergill JE, Murray GI (2001) Application of laser capture microdissection and proteomics in colon cancer. Mol Pathol 54:253–258PubMedCrossRefGoogle Scholar
  13. 13.
    Huang LE, Luzzi V, Ehrig T, Holtschlag V, Watson MA (2002) Optimized tissue processing and staining for laser capture microdissection and nucleic acid retrieval. Methods Enzymol 356:49–62PubMedCrossRefGoogle Scholar
  14. 14.
    Callagy G, Pharoah P, Chin SF, Sangan T, Daigo Y, Jackson L, Caldas C (2005) Identification and validation of prognostic markers in breast cancer with the complementary use of array-CGH and tissue microarrays. J Pathol 205:388–396PubMedCrossRefGoogle Scholar
  15. 15.
    Callagy G, Jackson L, Caldas C (2005) Comparative genomic hybridization using DNA from laser capture microdissected tissue. In: Murray GI, Curran S (eds) Methods in molecular biology vol 293: Laser capture microdissection methods and protocols. Humana, Totowa, NJ, pp 39–55Google Scholar
  16. 16.
    Rooney PH (2005) Multiplex quantitative real-time PCR of laser microdissected tissue. In: Murray G, Curran S (eds) Methods in molecular biology vol 293:Laser capture microdissection methods and protocols. Humana, Totowa, NJ, pp 27–37Google Scholar
  17. 17.
    Kheng GK, Cruickshank ME, Rooney PH, Miller ID, Parkin DE, Murray GI (2005) Human papillomavirus 16 infection in adenocarcinoma of the cervix. Br J Cancer 93:1301–1304CrossRefGoogle Scholar
  18. 18.
    Adeyi OA, Belloni DR, Dufresne SD, Schned AR, Tsongalis GI (2005) Real-time polymerase chain reaction and laser capture microdissection for the diagnosis of BK virus infection in renal allografts. Am J Clin Pathol 124:537–542PubMedCrossRefGoogle Scholar
  19. 19.
    Kerman IA, Buck BJ, Evans SJ, Akil H, Watson SJ (2006) Combining laser capture microdissection with quantitative real-time PCR: effects of tissue manipulation on RNA quality and gene expression. J Neurosci Methods 153:71–85PubMedCrossRefGoogle Scholar
  20. 20.
    Vincek V, Nassiri M, Block N, Welsh CF, Nadji M, Morales AR (2005) Methodology for preservation of high molecular-weight RNA in paraffin-embedded tissue: application for laser-capture microdissection. Diagn Mol Pathol 14:127–133PubMedCrossRefGoogle Scholar
  21. 21.
    Yang F, Foekens JA, Yu J, Sieuwerts AM, Timmermans M, Klijn JG, Atkins D, Wang Y, Jiang Y (2006) Laser microdissection and microarray analysis of breast tumors reveal ER-alpha related genes and pathways. Oncogene 25:1413–1419PubMedCrossRefGoogle Scholar
  22. 22.
    Luzzi V, Mahadevappa M, Raja R, Warrington JA, Watson MA (2003) Accurate and reproducible gene expression profiles from laser capture microdissection, transcript amplification, and high density oligonucleotide microarray analysis. J Mol Diagn 5:9–14PubMedCrossRefGoogle Scholar
  23. 23.
    Keays KM, Owens GP, Ritchie AM, Gilden DH, Burgoon MP (2005) Laser capture microdissection and single-cell RT-PCR without RNA purification. J Immunol Methods 302:90–98PubMedCrossRefGoogle Scholar
  24. 24.
    Ernst G, Melle C, Schimmel B, Bleul A, von Eggeling F (2006) Proteohistography-Direct analysis of tissue with high sensitivity and high spatial resolution using proteinchip technology. J Histochem Cytochem 54:13–17PubMedCrossRefGoogle Scholar
  25. 25.
    Gulmann C, Espina V, Petricoin E 3rd, Longo DL, Santi M, Knutsen T, Raffeld M, Jaffe ES, Liotta LA, Feldman AL (2005) Proteomic analysis of apoptotic pathways reveals prognostic factors in follicular lymphoma. Clin Cancer Res 11:5847–5855PubMedCrossRefGoogle Scholar
  26. 26.
    Caldwell RL, Caprioli RM (2005) Tissue profiling by mass spectrometry: a review of methodology and applications. Mol Cell Proteomics 4:394–401PubMedCrossRefGoogle Scholar
  27. 27.
    Guo J, Colgan TJ, DeSouza LV, Rodrigues MJ, Romaschin AD, Siu KW (2005) Direct analysis of laser capture microdissected endometrial carcinoma and epithelium by matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun Mass Spectrom 19:2762–2766PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Graeme I. Murray
    • 1
  1. 1.Department of PathologyUniversity Medical Buildings, University of AberdeenAberdeenUK

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