Enzyme Linked Immunosorbent Assay (ELISA)

  • John Crowther
Part of the Springer Protocols Handbooks book series (SPH)

1. Introduction

There have been very few developments that markedly affect the need to greatly revise the text from the last version of this book. This is testament to the fact that heterogeneous enzyme linked immunosorbent assays (ELISA) provide ideal systems for dealing with a wide range of studies in many biological areas. The main reason for this success is test flexibility, whereby reactants can be used in different combinations, either attached passively to a solid phase support or in the liquid phase. The exploitation of the ELISA has been increased through continued development of specifically produced reagents for example, monoclonal and polyclonal antibodies and peptide antigens coupled with the improvement and expansion of commercial products such as enzyme linked conjugates; substrates and chromogens; plastics technology and design of microwell plates; instrumentation advances and robotics. However, the principles of the ELISA remain the same.

A brief scan of the literature...


Avian Influenza Test Antibody Sandwich ELISA Indirect ELISA Plastic Surface 
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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • John Crowther
    • 1
  1. 1.International Atomic Energy AgencyViennaAustria

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