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Glycoprotein Analysis

  • Terry D. Butters
  • David C. A. Neville
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

1. Introduction

In eukaryotic cells, one of the most important post-translational modifications of proteins is the covalent addition of carbohydrate. We can consider two major types of modification to amino acid residues: N-glycosylation of asparagine amine side-chain groups and O-glycosylation of serine or threonine hydroxyl side-chain groups ( 1). An additional prerequisite of N-linked glycosylation is that the asparagine is part of the tripeptide sequon asparagine-X-serine/threonine (Asn-X-Ser/Thr) where X can be any amino acid except proline. N-Linked oligosaccharides can be divided into three major classes; the complex type containing N-acetylglucosamine, N-acetylgalactosamine, mannose, galactose, fucose, and sialic acid; the oligomannose type containing N-acetylglucosamine and mannose only, and the hybrid type that has features common to both complex and oligomannose chains (Fig. 31.1). All of these structures are synthesized by a common pathway that begins in the endoplasmic...

Keywords

Supercritical Fluid Chromatography Oligosaccharide Structure Glycoprotein Structure Exoglycosidase Digestion Oligosaccharide Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Terry D. Butters
    • 1
  • David C. A. Neville
    • 1
  1. 1.Glycobiology Institute, Department of BiochemistryOxford UniversityOxford, OxfordshireUK

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