Glycoprotein Analysis

  • Terry D. Butters
  • David C. A. Neville
Part of the Springer Protocols Handbooks book series (SPH)

1. Introduction

In eukaryotic cells, one of the most important post-translational modifications of proteins is the covalent addition of carbohydrate. We can consider two major types of modification to amino acid residues: N-glycosylation of asparagine amine side-chain groups and O-glycosylation of serine or threonine hydroxyl side-chain groups ( 1). An additional prerequisite of N-linked glycosylation is that the asparagine is part of the tripeptide sequon asparagine-X-serine/threonine (Asn-X-Ser/Thr) where X can be any amino acid except proline. N-Linked oligosaccharides can be divided into three major classes; the complex type containing N-acetylglucosamine, N-acetylgalactosamine, mannose, galactose, fucose, and sialic acid; the oligomannose type containing N-acetylglucosamine and mannose only, and the hybrid type that has features common to both complex and oligomannose chains (Fig. 31.1). All of these structures are synthesized by a common pathway that begins in the endoplasmic...


Supercritical Fluid Chromatography Oligosaccharide Structure Glycoprotein Structure Exoglycosidase Digestion Oligosaccharide Sequence 
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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Terry D. Butters
    • 1
  • David C. A. Neville
    • 1
  1. 1.Glycobiology Institute, Department of BiochemistryOxford UniversityOxford, OxfordshireUK

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