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Autoradiography and Fluorography

  • Bronwen M. Harvey
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

1. Introduction

Autoradiography is a technique widely used in biology and medicine. It uses a photographic emulsion to visualize molecules that are radioactively labeled. The technique provides a convenient method of detection, giving an accurate representation of the distribution of the label within a sample. The image is often referred to as the autoradiograph or autoradiogram.

The image is generated through the radioactive decay of the labeled ligand that is bound to a protein and/or nucleic acid found within a tissue or cell or held on an insert solid support (see Table 26.1). For example, it can be used to:
  • Identify a specific protein from a mixture separated by polyacrylamide electrophoresis (1) and held on a support: Western blotting (2).

  • Identify nucleic acid fragments separated by gel electrophoresis held on a support: Southern blotting (3), Northern blotting (4).

  • Identify the distribution of nucleic acids in a tissue section: in situ hybridization (5,6).

  • Identify the cellular...

Keywords

Photographic Emulsion Latent Image Silver Atom Silver Halide Silver Iodide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

Figures reproduced from a “Guide to Autoradiography” and Efficient detection of Biomolecules by Autoradiography, Fluorography and Chemiluminescence. Review booklet 23. GE Healthcare UK Ltd, Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, England.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Bronwen M. Harvey
    • 1
  1. 1.GE HealthcareMedical Diagnostics, Group CentreAmershamUK

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