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Gene Expression Profiling

  • Arnis Druka
  • Robbie Waugh
  • Pete Hedley
Protocol
  • 3.1k Downloads
Part of the Springer Protocols Handbooks book series (SPH)

1. Introduction

Gene expression profiling measures the relative abundance of a large number of individual mRNA species within the context of a total mRNA population that has been isolated and purified from a target biological sample. The technology for achieving this evolved from low-throughput membrane-based probe-to- target hybridization assays (northern and Southern blotting) commonly used in molecular biology labs since the 1980s (reviewed in 1). Scaling these assays down in size and up in number by redesigning the assay into a “target-to-probe” hybridization has enabled efficient and highly parallel genome-wide gene expression analysis. The technology platforms that emerged are generically called microarrays. A comprehensive review of microarrays, their history, application, and analysis is given by Schena (2).

The goal of gene expression profiling, achieved by microarray analysis, is to aid the biologist in identifying groups of genes that are functionally associated with certain...

Keywords

Microarray Experiment mRNA Abundance Gene Expression Matrix Dormancy Break Spotted Array 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Arnis Druka
    • 1
  • Robbie Waugh
    • 1
  • Pete Hedley
    • 1
  1. 1.Department of GeneticsSCRI InvergowrieDundeeUK

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