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Pronuclear Microinjection and Oviduct Transfer Procedures for Transgenic Mouse Production

  • Chengyu Liu
  • Wen Xie
  • Changyun Gui
  • Yubin Du
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1027)

Abstract

Transgenic mouse technology is a powerful method for studying gene function and creating animal models of human diseases. Currently, the most widely used method for generating transgenic mice is the pronuclear microinjection method. In this method, a transgenic DNA construct is physically microinjected into the pronucleus of a fertilized egg. The injected embryos are subsequently transferred into the oviducts of pseudopregnant surrogate mothers. A portion of the mice born to these surrogate mothers will harbor the injected foreign gene in their genomes. These procedures are technically challenging for most biomedical researchers. Inappropriate experimental procedures or suboptimal equipment setup can substantially reduce the efficiency of transgenic mouse production. In this chapter, we describe in detail our microinjection setup as well as our standard microinjection and oviduct transfer procedures.

Key words

Microinjection Pronuclear Transgenic Embryo transfer 

Notes

Acknowledgments

This work was supported by the Intramural Research Program of the National Heart, Lung, and Blood Institute.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Chengyu Liu
    • 1
  • Wen Xie
    • 1
  • Changyun Gui
    • 1
  • Yubin Du
    • 1
  1. 1.iPSC and Genome Engineering CoreNational Heart, Lung, and Blood Institute, National Institutes of HealthBethesdaUSA

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