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Dissecting the Cell Surface

  • Robert Fagan
  • Neil Fairweather
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 646)

Abstract

The bacterial cell surface is an important structure as it mediates interactions with the external environment. In the case of pathogens like Clostridium difficile, the cell wall and its components also have to mediate interactions with the host cells and their products. In this chapter we discuss the various methods used for dissecting the cell surface and the biochemical and immunological procedures that are commonly used to analyse the properties of the proteins within the cell wall. A major consideration is the S-layer which in C. difficile shows considerable variation in sequence and between strains, a property which is also reflected in its antigenic properties.

Key words

Cell wall S-layer protein purification ELISA immunofluorescence 

Notes

Acknowledgements

We thank Anne Wright, Omar Qazi and Natalie Welsh for developing and optimising some of the procedures described in this chapter.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Robert Fagan
    • 1
  • Neil Fairweather
    • 1
  1. 1.Centre for Molecular Microbiology and Infection, Imperial College LondonLondonUK

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