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Liposomes pp 51-75 | Cite as

Preparation of DRV Liposomes

  • Sophia G. AntimisiarisEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 605)

Abstract

Dried reconstituted vesicles (DRV) are liposomes that are formulated under mild conditions and have the capability to entrap substantially high amounts of hydrophilic solutes (compared with other types of liposomes). These characteristics make this liposome type ideal for entrapment of labile substances, as peptide, protein or DNA vaccines and sensitive drugs. In this chapter, we initially introduce all possible types of DRV liposomes (in respect to the encapsulated molecule characteristics and/or their applications in therapeutics) and discuss in detail the preparation methodologies for each type.

Key words

DRV Protein Peptide Hydrophilic drug Encapsulation yield Vaccine DNA Particulate Bacteria Cyclodextrin 

Abbreviations

BisHOP

1,2-Bis(hexadecylcycloxy)-3-trimethylaminopropane

CD

Cyclodextrin

CF

5,6-Carboxyfluorescein

CFA

Complete Freund’s adjuvant

Chol

Cholesterol

DMPC

1,2-Dimyristoyl-sn-glyceroyl-3-phosphocholine

DC-Chol

3b-(N,N-Dimethylaminoethane)carbamylcholesterol

DOTAP

1,2-Dioleyloxy-3-trimethylammonium propane

DOTMA

N-[1-(2,3-Dioleyloxy) propyl]-N,N,N-triethylammonium

DPPC

1,2-Dipalmitoyl-sn-glyceroyl-3-phosphocholine

DPPE-PEG2000

1,2-Dipalmitoyl-sn-glyceroyl-3-phosphoethanolamine conjugated to polyethylene glycol (MW 2000)

DRV

Dried rehydrated vesicles or Dried Reconstituted vesicles

DSPC

1,2-Distearoyl-sn-glyceroyl-3-phosphocholine

HPβ-CD

Hydroxypropyl-beta-CD

H-PC

Hydrogenated PC

Iv

Intravenous

MLV

Multilamellar vesicles

MW

Molecular weight

PA

Phosphatidic acid

PB

Phoshate buffer

PBS

Phosphate buffered saline

PC

Phosphatidylcholine

PCS

Photon correlation spectroscopy

PEG

Polyethylene glycol

PG

Phosphatidylglycerol

PRE

Prednisolone

PS

Phosphatidyl serine

RCM

Radiographic contrast media

rgp63

Recombinant glycoprotein of leishmania

SA

Stearylamine

SM

Sphingomyelin

SUV

Small unilamellar vesicles

Tc

Lipid transition temperature

TO

Triolein

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Laboratory of Pharmaceutical Technology, Department of PharmacyUniversity of Patras, and ICE-HT/FORTHPatrasGreece

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