Liposomes pp 51-75 | Cite as

Preparation of DRV Liposomes

  • Sophia G. AntimisiarisEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 605)


Dried reconstituted vesicles (DRV) are liposomes that are formulated under mild conditions and have the capability to entrap substantially high amounts of hydrophilic solutes (compared with other types of liposomes). These characteristics make this liposome type ideal for entrapment of labile substances, as peptide, protein or DNA vaccines and sensitive drugs. In this chapter, we initially introduce all possible types of DRV liposomes (in respect to the encapsulated molecule characteristics and/or their applications in therapeutics) and discuss in detail the preparation methodologies for each type.

Key words

DRV Protein Peptide Hydrophilic drug Encapsulation yield Vaccine DNA Particulate Bacteria Cyclodextrin 









Complete Freund’s adjuvant








1,2-Dioleyloxy-3-trimethylammonium propane


N-[1-(2,3-Dioleyloxy) propyl]-N,N,N-triethylammonium




1,2-Dipalmitoyl-sn-glyceroyl-3-phosphoethanolamine conjugated to polyethylene glycol (MW 2000)


Dried rehydrated vesicles or Dried Reconstituted vesicles






Hydrogenated PC




Multilamellar vesicles


Molecular weight


Phosphatidic acid


Phoshate buffer


Phosphate buffered saline




Photon correlation spectroscopy


Polyethylene glycol






Phosphatidyl serine


Radiographic contrast media


Recombinant glycoprotein of leishmania






Small unilamellar vesicles


Lipid transition temperature




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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Laboratory of Pharmaceutical Technology, Department of PharmacyUniversity of Patras, and ICE-HT/FORTHPatrasGreece

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