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Liposomes pp 29-50 | Cite as

Nanoliposomes: Preparation and Analysis

  • M. R. MozafariEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 605)

Abstract

Nanoliposome, or submicron bilayer lipid vesicle, is a new technology for the encapsulation and delivery of bioactive agents. The list of bioactive material that can be incorporated to nanoliposomes is immense, ranging from pharmaceuticals to cosmetics and nutraceuticals. Because of their biocompatibility and biodegradability, along with their nanosize, nanoliposomes have potential applications in a vast range of fields, including nanotherapy (e.g. diagnosis, cancer therapy, gene delivery), cosmetics, food technology and agriculture. Nanoliposomes are able to enhance the performance of bioactive agents by improving their solubility and bioavailability, in vitro and in vivo stability, as well as preventing their unwanted interactions with other molecules. Another advantage of nanoliposomes is cell-specific targeting, which is a prerequisite to attain drug concentrations required for optimum therapeutic efficacy in the target site while minimising adverse effects on healthy cells and tissues. This chapter covers nanoliposomes, particularly with respect to their properties, preparation methods and analysis.

Key words

Cancer therapy Food nanotechnology Gene delivery Mozafari method Nanoliposomes Nanotherapy 

Abbreviations

Chol

Cholesterol

DCP

Dicetylphosphate

DPPC

Dipalmitoyl phosphatidylcholine

EE

Entrapment efficiency

FFF

Field flow fractionation

HM-liposomes

Lipid vesicles prepared by the heating method

HPLC

High-performance liquid chromatography

OH

Hydroxyl group

LUV

Large unilamellar vesicles

MLV

Multilamellar vesicles

NMR

Nuclear magnetic resonance

PC

Phosphatidylcholine

SPM

Scanning Probe Microscopy

SUV

Small unilamellar vesicles

Tc

Phase transition temperature

TLC

Thin layer chromatography

Tm

Melting temperature

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Phosphagenics R&D LaboratoryClaytonAustralia

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