Helicases pp 359-376 | Cite as

Characterization of the Helicase Activity and Anti-telomerase Properties of Yeast Pif1p In Vitro

  • Jean-Baptiste Boulé
  • Virginia A. Zakian
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 587)

Abstract

Pif1p is the prototype member of a family of helicases that is highly conserved from yeast to humans. In yeast, Pif1p is involved in many aspects of the preservation of genome stability. In particular, Pif1p is involved in the maintenance of mitochondrial DNA and in the direct inhibition of telomerase at telomeres and double-stranded breaks. Here we describe methods to purify Pif1p and study in vitro its enzymatic properties and functional interaction with telomerase.

Key words

Yeast Pif1p helicase telomerase oligonucleotide substrate-based radiometric assays 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jean-Baptiste Boulé
    • 1
  • Virginia A. Zakian
    • 1
  1. 1.Department of Molecular BiologyPrinceton UniversityPrincetonUSA

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