Utilization of Multiple Real-Time PCR Assays for the Diagnosis of Bordetella spp. in Clinical Specimens

Part of the Methods in Molecular Biology book series (MIMB, volume 943)


Bordetella pertussis causes an upper respiratory infection in infants, adolescents, and adults. Diagnosis of pertussis, a vaccine-preventable disease, can be difficult, but recent implementation of real-time PCR assays in laboratories has hastened the ability of clinicians to make an accurate diagnosis. In this paper we describe the method of nasopharyngeal specimen collection, extraction of DNA, and real-time PCR assays that will allow the detection and identification of Bordetella spp. in clinical specimens.


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Division of Bacterial Diseases, Centers for Disease Control and Prevention, National Center for Immunization and Respiratory DiseasesMeningitis and Vaccine Preventable Diseases BranchAtlantaUSA

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