Image Analysis and Quantitative Morphology

  • Carlos Alberto Mandarim-de-Lacerda
  • Caroline Fernandes-Santos
  • Marcia Barbosa Aguila
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 611)

Abstract

Quantitative studies are increasingly found in the literature, particularly in the fields of development/evolution, pathology, and neurosciences. Image digitalization converts tissue images into a numeric form by dividing them into very small regions termed picture elements or pixels. Image analysis allows automatic morphometry of digitalized images, and stereology aims to understand the structural inner three-dimensional arrangement based on the analysis of slices showing two-dimensional information. To quantify morphological structures in an unbiased and reproducible manner, appropriate isotropic and uniform random sampling of sections, and updated stereological tools are needed. Through the correct use of stereology, a quantitative study can be performed with little effort; efficiency in stereology means as little counting as possible (little work), low cost (section preparation), but still good accuracy. This short text provides a background guide for non-expert morphologists.

Key words

Image analysis morphometry stereology volume density surface density length density disector 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Carlos Alberto Mandarim-de-Lacerda
    • 1
  • Caroline Fernandes-Santos
    • 1
  • Marcia Barbosa Aguila
    • 1
  1. 1.Laboratory of Morphometry and Cardiovascular MorphologyInstitute of Biology, Biomedical Center, State University of Rio de JaneiroRio de JaneiroBrazil

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