Image Analysis and Quantitative Morphology
Quantitative studies are increasingly found in the literature, particularly in the fields of development/evolution, pathology, and neurosciences. Image digitalization converts tissue images into a numeric form by dividing them into very small regions termed picture elements or pixels. Image analysis allows automatic morphometry of digitalized images, and stereology aims to understand the structural inner three-dimensional arrangement based on the analysis of slices showing two-dimensional information. To quantify morphological structures in an unbiased and reproducible manner, appropriate isotropic and uniform random sampling of sections, and updated stereological tools are needed. Through the correct use of stereology, a quantitative study can be performed with little effort; efficiency in stereology means as little counting as possible (little work), low cost (section preparation), but still good accuracy. This short text provides a background guide for non-expert morphologists.
Key wordsImage analysis morphometry stereology volume density surface density length density disector
- 1.Aherne, W.A. and Dunnill, M.S. (1982) Morphometry. Edward Arnold Publishers, London.Google Scholar
- 4.Torres, T.S., Silva, G.D., Aguila, M.B., Carvalho, J.J., and Mandarim-de-Lacerda, C.A. (2008) Effects of rosiglitazone (a peroxysome proliferator-activated receptor gamma agonist) on the blood pressure and aortic structure in metabolically programmed (perinatal low protein) rats. Hypertens. Res. 31, 965–975.PubMedCrossRefGoogle Scholar
- 7.Weibel, E.R. (1979) Stereological Methods. Practical Methods for Biological Morphometry. Academic Press, New York.Google Scholar
- 13.Gundersen, H.J.G., Bendtsen, T.F., Korbo, L., Marcussen, N., Moller, A., Nielsen, K., Nyengaard, J.R., Pakkenberg, B., Sorensen, F.B., Vesterby, A., and West, M.J. (1988) Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS 96, 379–394.PubMedCrossRefGoogle Scholar
- 15.Elias, H., Hyde, D.M., and Scheaffer, R.L. (1983) A Guide to Practical Stereology. Karger, Basel, Switzerland.Google Scholar