Analgesia pp 237-259

Part of the Methods in Molecular Biology book series (MIMB, volume 617)

Electrophysiological and Neurochemical Techniques to Investigate Sensory Neurons in Analgesia Research

  • Alexandru Babes
  • Michael J. M. Fischer
  • Gordon Reid
  • Susanne K. Sauer
  • Katharina Zimmermann
  • Peter W. Reeh
Protocol

Abstract

The primary afferent nociceptive neuron has recently attracted major research interest because of the cloning of very selectively expressed and well-conserved ion channel genes. All parts of the neuron, sensory terminals, axon and cell body, are accessible to validated research techniques in vitro using various isolated tissues or cells taken from laboratory animals. Single-unit recording and measuring stimulated calcitonin gene-related peptide (CGRP) release as well as patch-clamping and calcium imaging of cultured sensory neurons provide different kinds of information, and no model alone answers all questions. In combination, however, consistent results and complementary evidence form a solid basis for translational research to follow.

Key words

Patch clamp Calcium imaging Neuropeptide release CGRP Single fibre recording Dorsal root ganglion Trigeminal ganglion Primary culture Ion channels 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Alexandru Babes
    • 1
  • Michael J. M. Fischer
    • 2
  • Gordon Reid
    • 3
  • Susanne K. Sauer
    • 2
  • Katharina Zimmermann
    • 2
  • Peter W. Reeh
    • 2
  1. 1.Department of Physiology and BiophysicsUniversity of BucharestBucharestRomania
  2. 2.Institute of Physiology and Pathophysiology, University of Erlangen – NurembergErlangenGermany
  3. 3.Department of PhysiologyUniversity CollegeCorkIreland

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