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Bioluminescent Imaging of Transplanted Islets

  • Xiaojuan Chen
  • Dixon B. Kaufman
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 574)

Abstract

Bioluminescence imaging (BLI) modalities have been developed, refined, and used broadly in the study of small animal models of human biology and disease, including monitoring the fate of transplanted islets in vivo in real time. In order to advance our understanding of the pathophysiology and immunobiology of islet transplantation as they occur in living animals, islet grafts tagged with light-emitting luciferase can be implanted in a mouse islet transplantation model and assessed using in vivo BLI. We have utilized transgenic islets expressing the firefly luciferase as donor islets in syngeneic and allogeneic islet transplant mouse models for monitoring islets in vivo by BLI after they have been transplanted at different sites of the mice, including the intrahepatic site via portal vein injection. The sensitive and non-invasive BLI system allows better understanding of the dynamic fate of transplanted islets and the relationships among the islet mass that ultimately engrafts, the quality of graft function, and overall glucose homeostasis. It permits detection of early changes in islet graft function or mass due to rejection to prompt timely therapeutic intervention and change the fate of the graft. This chapter details some of the procedures for islet isolation, transplantation, and imaging as well as considerations of using the BLI system in the field of islet transplantation research.

Key words

Islet isolation islet portal transplantation in mice bioluminescence imaging 

Notes

Acknowledgment

This work was supported by National Institutes of Health Grant DK063565 (D. B. K.) and the Juvenile Diabetes Research Foundation.

References

  1. 1.
    Shapiro, A. M., Ricordi, C., Hering, B. J., Auchincloss, H., Lindblad, R., Robertson, R. P., Secchi, A., Brendel, M. D., Berney, T., Brennan, D. C., Cagliero, E., Alejandro, R., Ryan, E. A., DiMercurio, B., Morel, P., Polonsky, K. S., Reems, J. A., Bretzel, R. G., Bertuzzi, F., Froud, T., Kandaswamy, R., Sutherland, D. E., Eisenbarth, G., Segal, M., Preiksaitis, J., Korbutt, G. S., Barton, F. B., Viviano, L., Seyfert-Margolis, V., Bluestone, J., and Lakey, J. R. (2006) International trial of the Edmonton protocol for islet transplantation.[see comment]. N Engl J Med 355, 1318–1330.PubMedCrossRefGoogle Scholar
  2. 2.
    Chen, X., and Kaufman, D. B. (2004) Bioluminescence imaging of pancreatic islet transplants. Curr Med Chem 4, 301–308.Google Scholar
  3. 3.
    Chen, X., Zhang, X., Larson, C. S., Baker, M. S., and Kaufman, D. B. (2006) In vivo bioluminescence imaging of transplanted islets and early detection of graft rejection. Transplantation 81, 1421–1427.PubMedCrossRefGoogle Scholar
  4. 4.
    Virostko, J. M. (2003) Assessment of Pancreatic Islet Transplantation Using In Vivo Bioluminescence Imaging. Thesis Submitted to the Faculty of the Graduate School of Vanderbilt University.Google Scholar
  5. 5.
    Virostko, J., Chen, Z., Fowler, M., Poffenberger, G., Powers, A. C., and Jansen, E. D. (2004) Factors influencing quantification of in vivo bioluminescence imaging: application to assessment of pancreatic islet transplants. Mol Imaging: Official J Soc Mol Imaging 3, 333–342.Google Scholar

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Xiaojuan Chen
    • 1
  • Dixon B. Kaufman
    • 1
  1. 1.Division of Organ Transplantation, Department of Surgery, Feinberg School of MedicineNorthwestern UniversityChicagoUSA

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