G Protein-Coupled Receptors in Drug Discovery pp 199-211

Part of the Methods in Molecular Biology book series (MIMB, volume 552)

BacMam: Versatile Gene Delivery Technology for GPCR Assays

  • Elizabeth A. Davenport
  • Parvathi Nuthulaganti
  • Robert S. Ames

Summary

BacMam viruses are modified baculoviruses that contain mammalian expression cassettes for viral gene delivery and transient expression in mammalian cells. They are easily, inexpensively, and rapidly generated and provide a versatile solution for G protein-coupled receptor (GPCR) cell-based assay development. Using BacMam technology, target gene expression levels are easily controlled and simultaneous delivery of multiple genes is possible, for example, coexpression of a receptor and a G protein or a reporter gene. BacMam viruses are compatible with the GPCR cell-based assay formats typically used in high-throughput screening and provide an unparalleled level of experimental flexibility that is simply not possible when using stable recombinant cell lines.

Key words

BacMam Modified baculovirus Transient expression Viral transduction G protein-coupled receptors GPCR Cell-based assay 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Elizabeth A. Davenport
    • 1
  • Parvathi Nuthulaganti
    • 2
  • Robert S. Ames
    • 2
  1. 1.Molecular Discovery Research, Biological Reagents and Assay DevelopmentGlaxoSmithKline R&DCollegevilleUSA
  2. 2.Molecular Discovery Research, Biological Reagents and Assay DevelopmentGlaxoSmithKline R&DKing of PrussiaUSA

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