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Detergents and Chaotropes for Protein Solubilization Before Two-Dimensional Electrophoresis

  • Thierry Rabilloud
Part of the Methods in Molecular Biology™ book series (MIMB, volume 528)

Abstract

Because of the outstanding ability of two-dimensional electrophoresis to separate complex mixtures of intact proteins, it would be advantageous to apply it to all types of proteins, including hydrophobic and membrane proteins. Unfortunately, poor solubility hampers the analysis of these molecules. As these problems arise mainly in the extraction and isoelectric focusing steps, the solution is to improve protein solubility under the conditions prevailing during isoelectric focusing. This chapter describes the use of chaotropes and novel detergents to enhance protein solubility during sample extraction and isoelectric focussing, and discusses the contribution of these compounds to improving proteomic analysis of membrane proteins.

Key words

Hydrophobic protein membrane proteins protein solubilization isoelectric focusing chaotropes detergents zwitterionic detergents 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Thierry Rabilloud
    • 1
  1. 1.Laboratoire, d’immunologieDRDC/ICH, INSERMGrenobleFrance

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