The Isolation of scFvs Against Small Target Molecules
Phage display has the capacity to rapidly isolate recombinant antibodies against protein targets and other molecules of significant size. However, there is no obvious lower limit to the power of the selection methods: this chapter describes how the techniques of phage display can be adapted to allow the isolation of antibodies against very small compounds. Antibodies generated in this way have many uses including the detection and quantitative analysis of the target chemical moiety in samples such as foods, water, and body fluids.
Key words:Carriers Conjugation chemistry Biacore Hapten Pesticide Analyte
- 6.Hermanson, G. T. (1996) Bioconjugate Chemistry. Academic, London.Google Scholar
- 7.Yuan, B., Schulz, P., Liu, R., and Sierks, M. R. (2006) Improved affinity selection using phage display technology and off-rate based selection. Electron. J. Biotechnol. [online] 9, 2. Available from: http://www.ejbiotechnology.info/content/vol9/issue2/full/6/index.html. ISSN 0717-3458.CrossRefGoogle Scholar