The Isolation of scFvs Against Small Target Molecules

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 562)

Summary

Phage display has the capacity to rapidly isolate recombinant antibodies against protein targets and other molecules of significant size. However, there is no obvious lower limit to the power of the selection methods: this chapter describes how the techniques of phage display can be adapted to allow the isolation of antibodies against very small compounds. Antibodies generated in this way have many uses including the detection and quantitative analysis of the target chemical moiety in samples such as foods, water, and body fluids.

Key words:

Carriers Conjugation chemistry Biacore Hapten Pesticide Analyte 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.National Centre for Biomedical Engineering ScienceNational University of Ireland - GalwayGalwayIreland

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