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Analyzing Gene Function in Whole Mouse Embryo and Fetal Organ In Vitro

  • Satomi S. Tanaka
  • Yasuka L. Yamaguchi
  • Vanessa J. Jones
  • Patrick P. L. Tam
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1092)

Abstract

A well-established experimental paradigm to analyze gene function in development is to elucidate the impact of gain and loss of gene activity on cell differentiation, tissue modelling, organogenesis, and morphogenesis. This chapter describes the experimental protocols to study gene function by means of electroporation and lipofection to manipulate genetic activity in whole embryos and fetal organs in vitro. These techniques allow for more precise control of the timing, with reference to developmental age or stage, and the cell/tissue-specificity of the changes in gene activity. They provide an alternative strategy that can expedite the analysis of gene function before resorting to the conventional means of transgenesis and gene targeting in the whole organism.

Key words

Mouse embryo Fetal ovary In vitro culture Electroporation Lipofection siRNA 

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Satomi S. Tanaka
    • 1
  • Yasuka L. Yamaguchi
    • 1
  • Vanessa J. Jones
    • 2
  • Patrick P. L. Tam
    • 2
  1. 1.Department of Kidney Development, Institute of Molecular Embryology and GeneticsKumamoto UniversityKumamotoJapan
  2. 2.Children’s Medical Research Institute, Sydney Medical SchoolThe University of SydneySydneyAustralia

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