Two-Color In Situ Hybridization of Whole-Mount Mouse Embryos

  • Kristin K. Biris
  • Terry P. Yamaguchi
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1092)

Abstract

RNA in situ hybridization is a powerful technique used to identify the spatial localization of a specific RNA in a tissue section or whole tissue. In this protocol, we describe a reliable method for two-color in situ hybridization that can be used to accurately assess the expression of multiple genes with contrasting or overlapping expression patterns in whole mouse embryos.

Key words

In situ hybridization Development Embryo RNA probe Color detection Antibody 
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Notes

Acknowledgements

This research was supported by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research.

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Kristin K. Biris
    • 1
  • Terry P. Yamaguchi
    • 1
  1. 1.Frederick National Lab of Cancer Research, NCI, NIHFrederickUSA

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