Matrix-Assisted Laser Desorption Ionization Mass Spectrometry as a Complement to Internal Protein Sequencing

  • Kenneth R. Williams
  • Suzy M. Samandar
  • Kathryn L. Stone
  • Melissa Saylor
  • John Rush
Protocol

Abstract

Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is a relatively new mass-analysis method suitable for the analysis of biological molecules (1, 2, 3). Compared to other mass-analysis methods, MALDI-MS is based on simpler instrumentation that is more easily operated and so is more readily accessible to laboratories without prior experience in mass spectrometry. In addition, the method is sensitive and capable of high throughput. Its use in biochemical laboratories is already widespread, despite its recent introduction and the continued development of MALDI-MS instrumentation and applications.

Keywords

Tryptic Peptide Mass Accuracy Internal Sequencing Laser Desorption Ionization Mass Spectrometry Peptide Purity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Humana Press Inc., Totowa, NJ 1996

Authors and Affiliations

  • Kenneth R. Williams
    • 1
  • Suzy M. Samandar
    • 1
  • Kathryn L. Stone
    • 1
  • Melissa Saylor
    • 1
  • John Rush
    • 1
  1. 1.Biotechnology Resource LaboratoryYale UniversityNew Haven

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